实验方法库
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筛选
根据 “Protocols IO 数据源” 筛选结果如下：
Cell Culture and UV Cross-Linking
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
Exosome
RNA processing
Clémentine Delan-Forino, David Tollervey
2021-09-03
Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs
Jonathan Houseley, Cristina Cruz
2021-09-03
Mapping Exosome–Substrate Interactions In Vivo by UV Cross-Linking
Exosome
RNA processing
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
David Tollervey, Clémentine Delan-Forino
2021-09-03
Analysis of CRAC Datasets
RNA degradation
Protein–RNA interaction
RNA-binding sites
UV cross-linking
Yeast
Exosome
RNA processing
Clémentine Delan-Forino, David Tollervey
2021-09-03
Predicting Tankyrase Binders
Tankyrase
PARP
Poly(ADP-ribosyl)ation
Tankyrase-binding peptide motif
Enzyme–substrate relationships
Protein-protein interactions
Protein expression
Protein purification
Fluorescence polarization
FP
Structural biology
Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
2021-09-03
Validation of Tankyrase Binding and PARylation in Full-Length Protein Context
Tankyrase
PARP
Poly(ADP-ribosyl)ation
Tankyrase-binding peptide motif
Enzyme–substrate relationships
Protein-protein interactions
Protein expression
Protein purification
Fluorescence polarization
FP
Structural biology
Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
2021-09-03
Validating TBMs by Fluorescence Polarization (FP)
Tankyrase
PARP
Poly(ADP-ribosyl)ation
Tankyrase-binding peptide motif
Enzyme–substrate relationships
Protein-protein interactions
Protein expression
Protein purification
Fluorescence polarization
FP
Structural biology
Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
2021-09-03
Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach
Tankyrase
PARP
Poly(ADP-ribosyl)ation
Tankyrase-binding peptide motif
Enzyme–substrate relationships
Protein-protein interactions
Protein expression
Protein purification
Structural biology
Fluorescence polarization (FP)
Katie Pollock, Michael Ranes, Ian Collins, Sebastian Guettler
2021-09-03
qPCR Quantify cDNA, PCR, and Reaction Cleanup
Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt, Ashley L. Louie, Gene W. Yeo
2021-09-03
