实验方法库

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RNA-Seq

Transforming

Data analysis

CRISPR

Genetic

Explant Surgery: Chronic recoverable Neuropixels in mice

electrophysiology

silicon probe

electrode

Neuropixels

Freely moving recording: Chronic recoverable Neuropixels in mice

electrophysiology

silicon probe

behavior

position tracking

linear track

H-maze

Arduino

headfixed

spatial navigation

PhageFISH for DIG-labelled bacterial probes

PhageFISH

DIG-labelled bacterial probes

Preparation of Buffers for PhageFISH protocol

Permeabilisation buffer

rRNA hybridisation buffer

rRNA hybridisation wash buffer

rRNA CARD buffer

Gene hybridisation buffer

Gene hybridisation wash buffer

PhageFISH detailed protocol

Staining and embedding

CARD amplification

Phage probe hybridisation

Antibody binding

Quantification of various SARS-CoV-2 variant mutations (characteristic of Alpha, Beta, Gamma, Delta, Omicron and Omicron sublineages) in settled solids using digital RT-PCR

SARS-CoV-2

wastewater

alpha

beta

gamma

variant

mutation

COVID-19

Multiplexed snRNA-seq from frozen human brain samples

Nuclei isolation from frozen human brain samples for snRNA-seq

Immunofluorescence-based assay to assess LRRK2 recruitment by Rab29

ASAPCRN

LRRK2

Rab29

Golgi

immunofluorescence

616.1 URMC HTC BSL2+ Formalin-Inflated, Paraffin-Embedded Human Lung Tissue

Inflation fixation

lung

FFPE

COVID-19

SARS CoV2

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