List of IHC/WB antibodies, RT-PCR probes and software used
carmela giachino
Published: 2023-06-13 DOI: 10.17504/protocols.io.4r3l27ryjg1y/v1
Abstract
This protocol details list of antibodies used for immunohistochemistry and western blot.
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List of antibodies used for immunohistochemistry and western blot
1.
Table 1. List of antibodies used for immunohistochemistry and western blot.
| A | B | C | D |
|---|---|---|---|
| Antibody name | Company | Dilution | RRID |
| CCR2, goat polyclonal | Thermo Fisher Scientific | 1:500 | AB_557978 |
| CD11b, rabbit | Abcam | 1:1000 | AB_2650514 |
| CD11b, rat | Biolegend | 1:500 | AB_312784 |
| CD3, mouse | Santa Cruz Biotechnology | 1:500 | AB_2228831 |
| CD4, rat | Bio-Rad | 1:300 | AB_1898234 |
| CD68, rat | Bio-Rad | 1:300 | AB_2074849 |
| DAT, rabbit | Proteintech | 1:200 | AB_2879116 |
| GFAP, mouse | Sigma | 1:250 | AB_2827276 |
| GFAP, rabbit | sigma | 1:1000 | AB_2905668 |
| GSK-3β, mouse | ECM Biosciences | 1:200 | AB_2115216 |
| HuD+HuC, rabbit | Abcam | 1:400 | AB_2864321 |
| Iba-1, goat polyclonal | Abcam | 1: 200 | AB_870576 |
| Iba-1, rabbit polyclonal | Wako | 1:500 | AB_2889406 |
| iNOS, mouse | Cell signaling technology | 1: 200 | AB_1078202 |
| LRRK2, rabbit | clone 41-2, Abcam by MJFF | 1:1000 | AB_2713963 |
| MAP2, rabbit | Cell Signaling | 1:500 | AB_10693782 |
| NeuN, rabbit | Abcam | 1:1000 | AB_2716282 |
| Nurr1, goat polyclonal | R&D | 1:300 | AB_2153894 |
| phospho-alpha-Synuclein (Ser129), rabbit monoclonal (clone LS4-1B1) | Millipore | 1:1000 | AB_673008 |
| phospho-GSK-3β (p-Tyr216), rabbit | Abcam | 1:500 | AB_2533691 |
| Phospho-LRRK2-S935, rabbit | Abcam by MJFF | 1:1000 | AB_2864018 |
| phospho-Tau (pSer396), rabbit polyclonal | Sigma aldrich | 1:200 | AB_261757 |
| phospho-Tau (pThr181) | Thermo Fisher scientific | 1:200 | AB_1087704 |
| Tau, mouse | RayBiotech | 1:200 | AB_11217610 |
| TH, mouse | STEMCELL Technologies | 1:200 | AB_215512 |
| TH, rabbit | Pel-Freez | 1:200 | AB_2313713 |
| TH, sheep | Pel-Freez Biologicals | 1:200 | AB_2935637 |
| TH, sheep | Pel-Freez Biologicals | 1:1000 | AB_461070 |
| TUBB3, mouse | Biolegend | 1:1000 | AB_2313773 |
| α-syn (C-20)-R, rabbit | Santa Cruz Biotechnology | 1:200 | AB_2192953 |
| α-syn aggregate, MJFR-14-6-4-2, rabbit | Abcam | 1/5000 | AB_2714215 |
| β-actin, rabbit | Cell Signaling | 1:1000 | AB_10694076 |
2.
Table 2 . List of probes and IDs used for quantitative Real time PCR.
| A | B |
|---|---|
| PROBES | IDs |
| Casp6 | Mm00438053-m1 |
| CCl3 | Mm 00441258-m1 |
| CxCl10 | Mm 00445235-m1 |
| CxCl11 | Mm00444662-m1 |
| Drd1a | Mm01353211-m1 |
| Drd2 | Mm00438545-m1 |
| Foxa2 | Mm01976556-s1 |
| GAPDH | Mm99999915-g1 |
| Gfap | Mm 00546086-m1 |
| Gp91phox | Mm01287743-m1 |
| GSK3b | Mm00444911-m1 |
| IL-10 | Mm12088386-m1 |
| IL-17 | Mm00439618-m1 |
| IL-18 | Mm00434226-m1 |
| Il1b | Mm00434228-m1 |
| IL-4 | Mm00445259-m1 |
| Itgam (Mac1) | Mm 00434455-m1 |
| LRRK2 | Mm00481934_m1 |
| Map2 | Mm00485231-m1 |
| Nfkb1 | Mm00476361-m1 |
| Nos 2 | Mm 00440485-m1 |
| Nurr 1 | Mm 00443056-m1 |
| Slc6a3 (Dat) | Mm 00438388-m1 |
| Tau (Mapt) | Mm00521988-m1 |
| Th | Mm 00447546-m1 |
| Tnf | Mm00443258-m1 |
| Tnfrsf1b | Mm00441875-m1 |
| α-syn (SNCA) | Mm00458965-m1 |
3.
Table 3 List of software
| A | B | C |
|---|---|---|
| Software name | Version | URL |
| ImageJ | 1.53t | https://imagej.net/ |
| Luminex xPONENT | 4.3 | https://www.luminexcorp.com/xponent/ |
| Bio-Plex Manager | 6.2 | http://www.bio-rad.com/en-us/product/bio-plex-manager-software-standard-edition |
| ImageQuantity One | 4.6 | http://www.bio-rad.com/en-us/product/quantity-one-1-d-analysis-software |
| GraphPad Prism | 9 | http://www.graphpad.com/ |
